Telomerase regulation by the Pif1 helicase: a length-dependent effect?
نویسندگان
چکیده
منابع مشابه
The Pif1 Helicase, a Negative Regulator of Telomerase, Acts Preferentially at Long Telomeres
Telomerase, the enzyme that maintains telomeres, preferentially lengthens short telomeres. The S. cerevisiae Pif1 DNA helicase inhibits both telomerase-mediated telomere lengthening and de novo telomere addition at double strand breaks (DSB). Here, we report that the association of the telomerase subunits Est2 and Est1 at a DSB was increased in the absence of Pif1, as it is at telomeres, sugges...
متن کاملThe human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity
Telomeres, the protein-DNA complexes at the ends of eukaryotic chromosomes, are essential for chromosome stability, and their maintenance is achieved by the specialized reverse transcriptase activity of telomerase or the homologous recombination pathway in most eukaryotes. Here, we identified a human helicase, hPif1 that inhibits telomerase activity. The primary sequence and biochemical analysi...
متن کاملPif1 is a force-regulated helicase
Pif1 is a prototypical member of the 5' to 3' DNA helicase family conserved from bacteria to human. It has a high binding affinity for DNA, but unwinds double-stranded DNA (dsDNA) with a low processivity. Efficient DNA unwinding has been observed only at high protein concentrations that favor dimerization of Pif1. In this research, we used single-molecule fluorescence resonance energy transfer ...
متن کاملPif1 regulates telomere length by preferentially removing telomerase from long telomere ends
Telomerase, a ribonucleoprotein complex, is responsible for maintaining the telomere length at chromosome ends. Using its RNA component as a template, telomerase uses its reverse transcriptase activity to extend the 3'-end single-stranded, repetitive telomeric DNA sequence. Pif1, a 5'-to-3' helicase, has been suggested to regulate telomerase activity. We used single-molecule experiments to dire...
متن کاملSuppression of apoptosis by PIF1 helicase in human tumor cells.
Defining the processes that sustain telomere maintenance is critical to our understanding of cancer and longevity. PIF1 is a nonprocessive 5'->3' human DNA helicase that exhibits broad substrate specificity. In vitro studies have implicated PIF1 in maintaining telomeres and processing stalled DNA replication forks, but disruption of the murine Pif1 gene did not yield any apparent phenotype. In ...
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ژورنال
عنوان ژورنال: Current Genetics
سال: 2017
ISSN: 0172-8083,1432-0983
DOI: 10.1007/s00294-017-0768-6